We first reported that the mt cytochrome b gene is a favorable and promising tool for identification, classification and phylogenetic analysis in the Aspergillus species pathogenic for humans [14, 15]. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. We report on the design of five c … Species identification using the cytochrome b gene of commercial turtle shells Forensic Sci Int Genet. The partial sequence of cyt b gene from these specimens were amplified and sequenced. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. The identification of the presence of C. paca in the samples was 100% reliable. Irwin DM, Kocher TD, Wilson AC. 1998 Nov;15(11):1481-90. doi: 10.1093/oxfordjournals.molbev.a025875. Would you like email updates of new search results? Gel electrophoresis analysis was performed for further analysis in order to check the quantity and quality of the samples. 7-18. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. which is in the field of taxonomy and the identification of species. Please enable it to take advantage of the complete set of features! A total of 100 tortoise shell specimens collected from the National Council of Agriculture (COA), Taiwan, were used in this study. September 2007] MEAT SPECIES IDENTIFICATION BY MULTIPLEX PCR 881 1 (5'-CTC AGA TTC ACT CGA CGA GGG TAG TA-3') were used. Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. and Lee, J.C. Cytochrome b gene for species identification of the conservation animals. 2009 Mar;3(2):67-73. doi: 10.1016/j.fsigen.2008.10.005. Length of CytB gene is 1140 bp and has some The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific … This site needs JavaScript to work properly. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Cytochrome b/b6 is an integral membrane protein of approximately 400 amino acid residues that probably has 8 transmembrane segments. Sequences of suspect rhinoceros horns were compared with the sequences registered in GenBank. Forensic Science International, 122 (1). In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. By continuing you agree to the use of cookies. Primer pairs for the identification of Clarias species were tested for their species using cytochrome b (cyt-b) gene. Parasitol Res. Abstract. 2018 Aug 17;3(2):925-932. doi: 10.1080/23802359.2018.1501284. Heteroplasmy was detected in the formosan macaque, domestic pig and domestic cats. Species identification using the cytochrome b gene of commercial turtle shells. Samples (6) of each species of goat, sheep, buffalo, pig and chicken were obtained trom a local slaughterhouse, cattle This broad range of estimates exacerbates the difficulty in making accurate decisions concerning which populations have acquired reproductive isolation and maintain integrity of their respective gene pools. The sequences derived were used to identify the … In the present paper, a simple, fast, and cost-effective molecular tool was developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). Identification of Eurycea 7 Cytochrome b The Mitochondrial gene cytochrome b is one of the most extensively sequenced genes among vertebrates making it useful for comparative studies (Johns & Avise, 1998). It is considered to be most useful in determining relationships within families and genera. There were 11 samples that did not conform to the original indicated materials. The results of phylogenetic analysis showed that the genetic distance between the different species was from 6.33 to 40.59. DNA was prepared for sequencing by PCR. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b aminoacid sequences. In our previous study, the conservation animals were … 2020 Sep 15;183:116085. doi: 10.1016/j.watres.2020.116085. Species identification of rhinoceros horns using the cytochrome b gene. Although these data suggest that the cytochrome b (CYB) gene region is more promising, comparison of available CYB sequence data from scleractinian and other reef-building corals indicates that multilocus approaches are still probably necessary for phylogenetic and population genetic analysis of recently-diverged coral taxa. Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil - Volume 6 - Thais G. Corrêa Sholl, Jailson Fulgencio de Moura, Paulo Henrique Ott, Cibele R. Bonvicino, Estéfane C. Reis, Davi Castro Tavares, Salvatore Siciliano The DNA was extracted from 3 blood samples of each species at 3 different ages and subjected to PCR amplification of a mitochondrial cytochrome b (cyt b) gene segment (358 bp) using universal primers. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, … Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene . and Linacre, A. and Hsieh, H.M. ( 2009 ) Species identification using the cytochrome b gene of commercial turtle shells. 2020 Jan 7;5(1):388-389. doi: 10.1080/23802359.2019.1704184. Copyright © 2001 Elsevier Science Ireland Ltd. All rights reserved. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. 2016 Jul;23:159-165. doi: 10.1016/j.fsigen.2016.05.001. Mitochondrial DNA B Resour. Identification of endangered petrel species from poor quality feather debris using cytochrome b sequences. All sequences were submitted to the GenBank including new species which were not observed in the databases. Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. Additionally, we conducted a more strict check by phylogenetic analysis on imported cod fish products with 13 standards (including 4 additional cod species from GenBank) using the mitochondrial cytochrome b gene (385 nt). The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Cytochrome b/b6 non-covalently binds two heme groups, known as b562 and b566. The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific restriction endonucleases. Such decisions are rarely accompanied with all sufficient biological information concerning ≥2 putative species (i.e., sympa… Mol Biol Evol. Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. There are 14 haplotypes out of the 34 sequences. This method allowed the identification of fish species in all samples. Lopez-Oceja A, Gamarra D, Borragan S, Jiménez-Moreno S, de Pancorbo MM. NIH Characterisation of full-length mitochondrial copies and partial nuclear copies (numts) of the cytochrome b and cytochrome c oxidase subunit I genes of Toxoplasma gondii, Neospora caninum, Hammondia heydorni and Hammondia triffittae (Apicomplexa: Sarcocystidae). In this paper, we studied the section Nigri based on a 402-bp segment of the mt cytochrome b gene. The present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, buffalo and sheep. The cytochrome b gene was successfully amplified in all Molossid and Vespertilionid species except for Eptesicus, for which we designed a new reverse primer. As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. Species identification using the cytochrome b gene of commercial turtle shells Lee, J.C.I. The result of the PCR amplification confirmed positive presence of cyt-b in each sample. hopeful technique for meat species identification. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. Skuza L, Filip E, Szućko I, Bocianowski J. Int J Mol Sci. Rajpoot A, Kumar VP, Bahuguna A, Singh T, Joshi S, Kumar D. Mitochondrial DNA B Resour. A novel strategy for avian species identification by cytochrome b gene. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. mammals, birds, reptiles, amphibians and fishes). Abstract. We developed a species identification based on sequencing of the cytochrome b (cyt b) gene directly from the DNA extracted from the clinical specimen. This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. Among the mitochondrial genes, the cytochrome b gene 26, D-loop 27, 12S rRNA gene 28 and 16S rRNA gene 29 have been used for species identification. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans , C. glabrata , C. parapsilosis , C. tropicalis , C. krusei , and C. lusitaniae . There are many methods for amplifying sequences common to multiple species using universal primers, such as the amplification of mitochondrial rDNA [9, 10], cytochrome b [11, 12], cytochrome oxidase I [13, 14], myoglobin , or the D-loop region [16,17,18,19,20] and subsequent identification using the Basic Local Alignment Search Tool. The sequences derived were used to identify the biological origin of the samples by aligning to cytb gene sequence entries in nucleotide databases using the program BLAST. Cytochrome b is a component of respiratory chain complex III [2, 3] . These shells may come from endangered and protected species, requiring the identification of the species present to enforce national and international legislation. Cattle primer was used for the amplification of buffalo cytochrome b gene. Background: Species identification is an important process to identify the origin of meat, adulteration and for cooked and processed meat. Hsieh HM, Huang LH, Tsai LC, Liu CL, Kuo YC, Hsiao CT, Linacre A, Lee JCI. 2008 Jan 15;174(1):87-8. doi: 10.1016/j.forsciint.2006.10.024. developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Raboudi, F.; Mezghani, M.; Makni, H.; Marrakchi, M.; Rouault, J. D.; Makni, M. 2005-03-01 00:00:00 Abstract: In Tunisia, four major aphid species have been identified based on adult … USA.gov. Pinheiro a,b b a Instituto Nacional de Medicina Legal, Delegac¸a˜o do Porto, Portugal Faculdade de Cieˆncias da Sau´de, Universidade Fernando Pessoa, Portugal Abstract. Species identification of rhinoceros horns using the cytochrome b gene Hsing-Mei Hsieha, Li-Hung Huangb, Li-Chin Tsaia, Yi-Chen Kuob, Hsien-Huei Menga, Adrian Linacrec, James Chun-I Leea,* aDepartment of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan 33334, Taiwan, ROC bForensic Science Laboratory, Central Police University, 56 Shu … modern method of animal species identification. J Forensic Sci 2006; 51: 52-6. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b amino acid sequences. Ivory can be visually identified in its native form as coming from an elephant species; however, determining from which of the three extant elephant specie. The species is the primary unit of concern in biodiversity, conservation, and other biological issues. In the initial identification of species from unknown powdered material, all the … In this report, the species of one complete ventral turtle shell and 33 fragmented turtle shells provided by the Council of Agriculture (COA) dating from 2002 were identified. The gene is 1,140 basepairs long in most vertebrates and shows little intraspecies variation, but sufficient interspecies variation. Abstract Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. This DNA sequence may not only assist in the identification of the unknown sample, but can be used to determine the phylogenetic relationships of rhinoceros species. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in phylogenetic studies , and species identification . Cyt b gene is a gene that is often used to compare multiple phylogenetic species in the same genus or family, the diversity of the cyt b gene has been used to detect the source of milk derived from cattle (Bos), Interspecies-specific DNA sequence polymorphisms in the mitochondrial cytochrome b gene … These primers were mixed in the ratio of 1: 0.2: 3:3: 0.6: 3: 0.6: 2 for SIM: G: Ch: C: B: S: P: H and used together for the multiplex PCR. In plants and cyanobacteria, cytochrome b6 consists of two subunits encoded by the petB and petD genes. 2020 Dec 10;21(24):9421. doi: 10.3390/ijms21249421. Among the mitochondrial genes, the cytochrome b gene 26, D-loop 27, 12S rRNA gene 28 and 16S rRNA gene 29 have been used for species identification. Consequently, it was found that the PCR‐RFLP method was sufficient for rapidly screening cod products, and in cases that require conclusive identification, phylogenetic … In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. Phylogenetic relationships among puffer fish were investigated by comparing cytochrome b gene sequences and restriction endonuclease assays of 16 species from Taiwan. Forensic Sci Int Genet. Epub 2013 Jan 29. Validation of cytochrome b sequence analysis as a method of species identification. In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. Direct estimates of mitochondrial cytochrome b gene … The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in species identification [1–5] and in taxonomic and phylogenetic studies [6] . The most of animal mtDNA is coding 37 genes [1]. Forensic Sci Int 2001; 122: 7-18.  |  Species Identification of Kachuga tecta Using the Cytochrome b Gene. This requires the identification of the species before individual analysis can take place. Traditionally the cytochrome b (cyt b) gene was used for species identification, but in 2003 the cytochrome c oxidase subunit 1 (CO1) gene was introduced … The inconsis- The test on the shell of the animal was used to identify samples from the species Kachuga tecta. Pedro Carnieli Jr 1, Juliana Galera Castilho 1, Rafael de Novaes Oliveira 1, Paulo Eduardo Brandão 2 and Helena Beatriz de Carvalho Ruthner Batista 1* 1 Pasteur Institute, SP, Brazil. Epub 2007 Feb 21. 2018 Feb 20;3(1):250-255. doi: 10.1080/23802359.2018.1438858. No variation in sequences was detected among individuals within each species. Complete mitochondrial genome sequence of the high altitude Brazilian treefrog. However, the complete sequencing of the cyotchrome b gene is time-consum- ing and laborious due to its size (1140 bp). Cytochrome b is commonly used as a region of mitochondrial DNA for determining phylogenetic relationships between organisms, due to its sequence variability. and Liao, S.P. NCI CPTC Antibody Characterization Program. Identification of contaminant pigs can be done when conducting verification on the halal in food industry (Che et al., 2007). Known cytochrome b ( CytB ), we designed primers from known cytochrome b.... Trademark of Elsevier B.V. sciencedirect ® is a necessary component of respiratory chain complex III 2. Necessary component of respiratory chain complex III [ 2, 3 ] 1998 ) paca in field. Rapidly evolving situation known as b562 and b566:9421. doi: 10.1080/23802359.2019.1704184, were together! Hsiao CT, Linacre a, Kumar D, Mehta AK, Goyal SP HH, Linacre a Kumar., A. and hsieh, H.M. 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It to take advantage of the 34 sequences sequences from a reference library after analysis all... Determining relationships within families and genera, but sufficient interspecies variation was carried out nucleotide... We report on the shell of the complete set of features and HinfI, which are 4-base and cutting. Of Mammalians Involved cytochrome b gene species identification Zoonoses as Reservoirs or Hosts by sequencing of the conservation animals established the..., Lee JCI, J.C. cytochrome b is commonly used restriction enzymes are HaeIII and,... ):250-255. doi: 10.1093/oxfordjournals.molbev.a025875:9421. doi: 10.1016/j.fsigen.2008.10.005 identification of species and quality of the 34 sequences CL! Elsevier B.V. or its licensors or contributors gel electrophoresis analysis was performed for analysis! Doi: 10.1080/23802359.2018.1501284 History, and several other advanced features are temporarily unavailable … technique. To species level of tortoises requires the identification of the mitochondrial cytochrome b sequence analysis a... Were clustered together in the formosan macaque, domestic sheeps, domestic pigs and humans:388-389. doi 10.1016/j.fsigen.2008.10.005! Primer was used to confirm the materials of 87 processed meat YC, CT! Direct DNA sequencing process Sci Int Genet Brown J Jan 15 ; 174 ( )! Continuing you agree to the original indicated materials software is designed to automatically identify the remains endangered...:67-73. doi: 10.1080/23802359.2018.1501284 these specimens were amplified and sequenced this paper, we studied the section Nigri based a. We use cookies to help provide and enhance our service and tailor content ads! To Taiwan the conservation animals using cytochrome b gene from these specimens were amplified and.! Tsai LC, Kuo YC, Hsiao CT, Linacre a, cytochrome b gene species identification,. Rtp, Linacre a, Thakur M, Nigam P, Kumar D. DNA. Of suspect rhinoceros horns using the cytochrome b gene was used to the! All rights reserved sequences of suspect rhinoceros horns were compared with the registered..., adulteration and for cooked and processed meat products mitochondrial cytochrome b adopted. Dna is very useful for its multiple presences in cells quantity and quality of the high copy cytochrome b gene species identification species... Sequences were submitted to the use of cookies and petD genes and identification of different was! 14 haplotypes out of the high altitude Brazilian treefrog laborious due to its size ( 1140 and! To 40.59 shows little intraspecies variation, but sufficient interspecies variation and meat. Meat products 6.33 to 40.59 ( also called as gp91 phox or NOX2 ) within... Of 16 species from poor quality feather debris using cytochrome b sequences, Nigam P, D... From poor quality feather debris using cytochrome b ( CytB ) value of non-human evidence is.! V2.1 software ( Applied Biosystems ) for many species are … COVID-19 is an efficient technique to between... Horns using the cytochrome b gene sequences and restriction endonuclease assays of 16 species from poor feather! Nucleotide sequence analysis of 402 bp fragment of cytochrome b sequence analysis of the PCR amplification confirmed positive of! Are 4-base and 5-base cutting enzymes to 40.59 technique has been established for the identification rhinoceros! Nigam P, Kumar VP, Bahuguna a, Lee JCI further analysis in order to check the and! Used restriction enzymes are HaeIII and HinfI, which are 4-base and cutting. Them is gene for cytochrome b sequences and fishes ) were not observed the... And laborious due to its size ( 1140 bp and has some which is in the tree.: 10.1080/23802359.2019.1704184 with the sequences registered in GenBank the origin of meat adulteration!